Plasma Protein Production by Human Tumors Xenotransplanted in Nude Mice1

For detection of plasma proteins produced by human malignant tumors, a survey of blood plasma obtained from nude mice bearing serially transplanted human tu mors was performed by immunoelectrophoresis and the double immunodiffusion technique. Among 34 lines in cluding 18 types of human tumors, human specific plasma proteins were demonstrated in the plasma of nude mice transplanted with two lines of renal cell carcinoma, one adenocarcinoma of the colon, and one squamous cell carcinoma of the maxillary sinus. These tumors can be designated as "ectopie" plasma protein-producing tumors since the organs or tissues from which they originated are not considered to be usual sites of plasma protein synthe sis. Plasma protein production, as well as that of a,fetoprotein, was also found in one line of hepatoblasotma and three lines of yolk sac tumors. The above tumors were shown to produce one or more of the following 10 of 20 plasma proteins examined: albumin, prealbumin, a,-antitrypsin, ceruloplasmin, «.,macroglobulin, hemopexin, haptoglobin, C3 and C4 com ponent of complement, and transferrin. An immunochemical demonstration of human specific cancer products observed in human tumors xenotransplanted into nude mice may provide a new approach for investigating the metabolism of neoplastic cells.